Paraspeckles are mammalian subnuclear bodies that are built on the long noncoding RNA, NEAT1 (nuclear paraspeckle assembly transcript 1). Paraspeckles are induced with many different stressors and regulate gene expression by the subnuclear sequestration, or ‘sponging’ of specific transcription factors and the nuclear retention of some types of RNA. Within paraspeckles, the long 23kb isoform of NEAT1 has a distinct organisation such that the 5’ and 3’ ends are found at the periphery and the middle is found at the centre. I will present our latest super-resolution (structured illumination) microscopy data showing how distinct paraspeckle proteins and RNA molecules are arranged within these bodies. Further, I will describe how different NEAT1 isoforms have distinct arrangements within paraspeckles and within the cell nucleus.