Microorganisms isolated from various sources are known to have beneficial effects related to human health. Studies about such microorganisms have been conducted with various targets and purposes. However, the studies that targeted stem cells are rarely reported. This study aims to find out the effect of microorganism extracts on the property of murine skin-derived stem cells (mSKPs) such as sphere formation and proliferation rate. The extracts of various microorganisms were prepared individually and protein levels were measured to determine standard concentration of treatment. Firstly, mSKPs were treated with 100 ㎍/ml and 300 ㎍/ml of each extract for 3 days. Secondly, the effect of the treatment was confirmed with the morphology and proliferation rate of mSKP spheres. Then the 4 lines that have distinct effects compared to control group were selected and analyzed. The morphology of spheres in each group except for line 4 was significantly different from control group. At 100 ㎍/ml of extract, line 1, 2 and 3 had increased in size of spheres compared to control group. However, line 4 had no difference compared to control group. At 300 ㎍/ml, sphere size of line 1 and 2 got bigger than 100 ㎍/ml groups. In line 3, however, treatment of 300 ㎍/ml extract had a stronger effect on the increase of sphere size. Line 4 had no increase in size of spheres compared to control group. On the aspect of proliferation rate, line 1, 2 and 3 showed higher proliferation rate than control. Line 3 was the highest group, and line 1 and 2 followed next. Line 4 was even lower than the control group. According to our experiments, the results suggest that specific microorganism extracts can improve the sphere formation and proliferation of mSKPs. However, the accurate mechanisms that regulate these phenomenons are remained to be performed in the future. This study was supported by a grant from the National Research Foundation of Korea (No. 2016931864), Korea.