IMAGING CHROMATIN DYNAMICS DURING THE DNA DAMAGE RESPONSE — ASN Events
  1. Schedule
  2. Session 13: Imaging Workshop 1
  3. IMAGING CHROMATIN DYNAMICS DURING THE DNA DAMAGE RESPONSE

IMAGING CHROMATIN DYNAMICS DURING THE DNA DAMAGE RESPONSE (#47)

Belinda Wright 1 , Tara K Bartolec 2 , Tony Cesare 2 , Elizabeth Hinde 1
  1. EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW, Australia
  2. Genomic Integrity Group, Childrens Medical Research Institute, Sydney, NSW, Australia

Chromatin dynamics modulate DNA repair factor accessibility throughout the DNA damage response. The spatiotemporal scale upon which these dynamics occur render them invisible to live cell imaging1. Here we employ fluorescence lifetime imaging microscopy (FLIM) for FRET detection of nucleosome arrangement in live cells and monitor the structural rearrangements of chromatin during DNA repair. With this technology we demonstrate that genomic double strand breaks (DSBs) induce both local and global condensation events in the chromatin network and the detected chromatin dynamics appear to facilitate DNA repair factor recruitment specifically to the lesion site.